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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Gut. 2018 Jun 22;68(6):1003–1013. doi: 10.1136/gutjnl-2018-316226

Fig. 4. Depletion of macrophages with anionic liposomal clodronate blocks LGG-induced MSC migration and LGG radioprotection.

Fig. 4.

(A) Immunofluorescence for F4/80 expressing macrophages compares placebo treated mice having normal macrophage population with anionic clodronate liposome treated mice showing macrophage depletion. Dotted yellow line separates crypt and villus zones. (B) Cell counts provide quantitative data showing that treatment of mice with clodronate blocks LGG induced migration of COX2 expressing MSCs from the villus to the crypt zone in the small intestine. Data are means ± SEM for 5 to 7 mice per treatment group. ***P<.0001 compared with controls. (C) Crypt survival is significantly improved in LGG treated mice, and depletion of macrophages by treatment with clodronate blocks LGG induced radioprotection. Clodronate liposome (7mg/ml) were given i.p. at 4 days before (200μl/20g mouse), and again at 2 days before (100μl/20g mouse), and finally at eight hours before receiving 12Gy TBI. Data are means ± SEM for 5 to 7 mice per treatment group. **P<.001, ***P<.0001 compared with controls.