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Experimental phase memory curves for dual-spin-labeled Q1-VSD mutants (Phe123/Ser143) bearing two MTSL spin labels for liposomes (POPC/POPG = 3:1) (A) and POPC/POPG lipodisq nanoparticles (B). Tm values are 1.2 ± 0.2 μs for proteoliposomes and 2.0 ± 0.2 μs for lipodisq nanoparticles.
