Table 1.
Rotational Correlation Times (τ) and Relative Population (P) of Two Spectral Components of the EPR Spectra (Figure 4) Obtained from the Best-Fit NLSL MOMD Spectral Simulationa
liposomes | lipodisq | ||||||||
---|---|---|---|---|---|---|---|---|---|
sites | temperature (K) | τ, ns (site 1) | τ, ns (site 2) | P, % (site 1) | P, % (site 2) | τ, ns (site 1) | τ, ns (site 2) | P, % (site 1) | P, % (site 2) |
F130C | 297 | 23 | 1 | 63 | 37 | 57 | 1.8 | 73 | 27 |
325 | 1.1 | 1.0 | 33 | 67 | 34 | 0.8 | 92 | 8 | |
V165C | 297 | 17 | 1.1 | 64 | 36 | 58 | 2.3 | 63 | 37 |
325 | 1.8 | 0.8 | 77 | 23 | 18 | 1.0 | 82 | 18 | |
Q147C | 297 | 19 | 1.5 | 58 | 42 | 54 | 1.9 | 72 | 28 |
325 | 1.6 | 1.0 | 13 | 87 | 15 | 1.0 | 82 | 18 |
The uncertainty in the correlation time (τ) value is ±0.5−2 ns for liposome samples and ±0.5−5 ns for lipodisq samples, respectively. Similarly, the uncertainty in the relative population (P) value is ±3−6% for liposome samples and ±2−4% for lipodisq samples. These uncertainties were estimated based on the multiple batches of sample preparation and multiple simulations runs.