Table 2.
Peptide fragments generated from a time-course digestion of T1L virions
| Time (min) | σ3 | m/z (Obs.) | m/z (Calc.) | μl | m/z (Obs.) | m/z (Calc.) |
|---|---|---|---|---|---|---|
| A: Tryptic fragments (pH 7.4)a | ||||||
| 0.5 | — | — | — | 695–703 | 994.535 | 994.532 |
| 1.0 | — | — | — | 695–704 | 1150.635 | 1150.633 |
| 1.5 | 214–236 | 2684.226 | 2684.230 | — | — | — |
| 2.0 | 203–208 | 766.383 | 766.385 | — | — | — |
| 2.5 | — | — | — | — | — | — |
| 3.0 | 209–213 | 625.290 | 625.295 | — | — | — |
| 248–259 | 1422.661 | 1422.655 | — | — | — | |
| 241–259 | 2189.091 | 2189.089 | — | — | — | |
| 5.0 | 1–22b | 2511.210 | 2469.202 | — | — | — |
| 67–72 | 796.438 | 796.433 | — | — | — | |
| 79–90 | 1492.692 | 1492.682 | — | — | — | |
| 91–97 | 897.510 | 897.506 | — | — | — | |
| 79–97 | 2371.167 | 2371.170 | — | — | — | |
| 73–97 | 3113.515 | 3113.524 | — | — | — | |
| 98–122 | 2849.318 | 2849.321 | — | — | — | |
| 123–143 | 2392.166 | 2392.161 | — | — | — | |
| 187–196 | 1099.538 | 1099.528 | — | — | — | |
| 187–202 | 1740.846 | 1740.842 | — | — | — | |
| 209–236 | 3290.496 | 3290.506 | — | — | — | |
| 240–259 | 2317.191 | 2317.184 | — | — | — | |
| 241–259 | 2189.091 | 2189.089 | — | — | — | |
| 248–259 | 1422.661 | 1422.655 | — | — | — | |
| 268–281 | 1455.686 | 1455.680 | — | — | — | |
| 297–310 | 1581.841 | 1581.839 | — | — | — | |
| 297–312 | 1851.033 | 1851.024 | — | — | — | |
| 298–312 | 1722.922 | 1722.929 | — | — | — | |
| 313–326 | 1542.721 | 1542.716 | — | — | — | |
| 10.0 | 23–63 | 4326.029 | 4326.031 | — | — | — |
| 35–63 | 2962.389 | 2962.390 | — | — | — | |
| 98–120 | 2594.166 | 2594.151 | — | — | — | |
| 144–162 | 2156.049 | 2156.051 | — | — | — | |
| 327–365 | 4199.064 | 4199.083 | — | — | — | |
| 20.0 | 282–287 | 798.420 | 798.422 | 235–243 | 984.617 | 984.621 |
| 23–34 | 1382.662 | 1382.659 | — | — | — | |
| 214–234 | 2471.119 | 2471.108 | — | — | — | |
| 25.0 | 163–186 | 2873.360 | 2873.368 | — | — | — |
| B: Glu-C digested fragments (pH 7.4)a | ||||||
| 0.5 | — | — | — | — | — | — |
| 1.0 | — | — | — | — | — | — |
| 1.5 | — | — | — | — | — | — |
| 2.0 | — | — | — | — | — | — |
| 2.5 | — | — | — | 513–525 | 1374.728 | 1374.716 |
| 3.0 | — | — | — | — | — | — |
| 5.0 | 199–217 | 2188.044 | 2188.038 | — | — | — |
| 10.0 | — | — | — | — | — | — |
| 20.0 | 228–241 | 1685.878 | 1685.872 | — | — | — |
| 230–241 | 1443.750 | 1443.746 | — | — | — | |
| 30.0 | 21–30 | 1109.565 | 1109.559 | 137–153 | 1923.929 | 1923.916 |
| 50.0 | 3–20 | 1995.994 | 1995.996 | — | — | — |
| Time (min) | Glu-C (pH 4.5) | Pepsin (pH 3.0)a | ||||
| σ3 | m/z (Obs.) | m/z (Calc.) | σ3 | m/z (Obs.) | m/z (Calc.) | |
| C: Glu-C and pepsin digested fragments (pH 4.5 and 3.0) | ||||||
| 0.5 | — | — | — | — | — | — |
| 1.0 | — | — | — | — | — | — |
| 1.5 | — | — | — | 111–128 | 1958.942 | 1958.942 |
| 2.0 | — | — | — | 195–206 | 1419.741 | 1419.734 |
| 2.5 | — | — | — | 142–154 | 1573.778 | 1573.776 |
| 142–159 | 2115.057 | 2115.051 | ||||
| 3.0 | — | — | — | 27–42 | 1763.804 | 1763.791 |
| — | — | — | 245–265 | 2343.204 | 2343.200 | |
| — | — | — | 295–309 | 1708.903 | 1708.913 | |
| — | — | — | 343–357 | 1735.783 | 1735.789 | |
| 5.0 | 199–217 | 2188.036 | 2188.038 | Too many | ||
| 10.0 | — | — | — | |||
| 20.0 | 228–241 | 1685.873 | 1685.872 | |||
| 230–241 | 1443.740 | 1443.746 | ||||
| 30.0 | 21–30 | 1109.550 | 1109.559 | |||
| 50.0 | 3–20 | 1995.994 | 1995.996 | |||
| 60.0 | — | — | — | |||
| 120.0 | 176–198 | 2619.241 | 2619.245 | |||
| 301–309 | 998.464 | 998.470 | ||||
a
Assignments of all peaks corroborated by tandem MS.
b
Acetylation at the N-terminus.