Skip to main content
. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Sci Signal. 2015 Nov 3;8(401):ra109. doi: 10.1126/scisignal.aac5356

Fig. 4. Stable knockdown of ADAM17 prevents increased TNFR shedding and resistance to TNF.

Fig. 4.

(A) Immunoblot for pro (P) and mature (M) ADAM17 in lysates from L-929 cells expressing full-length and ΔN-iRhom2 transfected with either a scrambled control (Scr1) or an ADAM17-targeted shRNA (ADAM17-Sh1). Blot is representative of three experiments. (B) Surface abundance of TNFR1 and TNFR2 as determined by flow cytometry on L-929 cells expressing a vector control, full-length (WT) iRhom2 or iRhom2-ΔN in the presence of either control or ADAM17 shRNA (n = 6). (C and D) Cell death as a proportion of annexin–7-AAD+ cells in cultures of 1 × 105 L-929 cells stably expressing full-length or ΔN-iRhom2 (C) or iRhom1 (D) and either scrambled or ADAM17 shRNA treated with recombinant TNF for 48 hours (n = 4 or 6, respectively). Data are means ± SEM from the number of experiments (n) indicated; *P < 0.05, ***P < 0.001. MFI, mean fluorescence intensity.