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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: FASEB J. 2019 Nov 19;34(1):107–121. doi: 10.1096/fj.201901600R

FIGURE 5.

FIGURE 5

Generation and validation of BAT-specific KO of Kiss1r mice (“BAT cKO”). A, Strategy for selectively knocking out Kiss1r from BAT tissue by crossing Ucp1-Cre and Kiss1r floxed mouse lines. B, PCR product of different tissues collected from Ucp1-Cre+/Kiss1rfl/fl (BAT cKO) mice tested for Kiss1r gene recombination. C, Cross section of BAT from Ucp1-Cre−/Tdtomato+/− (control) and Ucp1-Cre+/TdTomato+/− (recombined) mice demonstrating fluorescent reporter only in the latter. D, Cross section of brain tissue from Ucp1-Cre−/Tdtomato+/− (control) and Ucp1-Cre+/Tdtomato+/− (recombined) mice demonstrating a few fluorescent cells in the cortex of the latter (a region of the brain where Kiss1r is not expressed). E, Gonad weights from adult BAT cKO males and females (n = 5–8/group for males and n = 11/group for females). F, AGD in adult BAT cKO males and females (n = 7–11/group for males and n = 12–13/group for females)