Figure 8.

TPO demonstrated a protective effect in a CoCl₂-induced PC12 cell injury protection model. Cell viability was detected by MTT. Cell apoptosis and mitochondrial membrane potential were detected by flow cytometry. (A) Effect of different concentrations of CoCl₂ on viability of PC12 cells. Cells were treated with CoCl₂ for 24 h, n = 3. (B) Protective effect of different concentrations of TPO on CoCl₂-induced (500 μmol/L) PC12 cells. Cells were treated with TPO for 48 h, n = 3. (C) Protective effect of 100 ng/mL of TPO on CoCl₂-induced (500 μmol/L) PC12 cells. (D) Protective effect of 100 ng/mL of TPO on mitochondrial membrane potential of CoCl₂-induced (500 μmol/L) PC12 cells. ** P < 0.01.