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. 2020 Apr 21;12(8):7282–7298. doi: 10.18632/aging.103076

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Copyright © 2020 Yao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Expression and correlation of HMGN5 and Hsp27 in tissue samples. (A) Pathological characteristics of normal and bladder cancer tissues assessed by HE staining. (B) Protein-protein-interaction analysis showing proteins related to HMGN5 based on data from BioGRID (https://thebiogrid.org/). (C, D) The expression of Hsp27 and HMGN5 in 56 paired normal and bladder cancer tissues examined by qPCR. (E) The correlation of Hsp27 and HMGN5 mRNA expression in tissue samples analyzed by Pearson’s correlation analysis. (F) The protein levels of Hsp27 and HMGN5 in tissue samples were examined by immunoblotting. (G) The protein levels and localization of Hsp27 and HMGN5 in tissue samples were examined by IHC staining. (H–J) The association of Hsp27 and HMGN5 expression with overall survival was analyzed by Kaplan-Meier overall survival analysis.