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. 2020 Apr 26;12(8):7248–7261. doi: 10.18632/aging.103073

Figure 2.

Figure 2

MiR-375 targeting ATG2B.3’-UTR. (A) miR-375 targeting the 3’-UTR of ATG2B predicted by TargetScan; (B) miR-375 mimics significantly reduced the wild 3’-UTR luciferase reporter activity of ATG2B; (C) OA chondrocytes were transfected with miRNA-375 mimics or inhibitor, and miRNA-375 expression was detected by qRT-PCR; (D, E) OA chondrocytes were transfected with pcDNA3.1-ATG2B, and ATG2B protein was detected by western blot, and the statistical results were presented; (F, G) protein expression of ATG2B, Collagen II and MMP13 were measured by western blot, and the statistical results were presented. * P<0.05, ** P<0.01, compared with NC group; #, P<0.05, compared with pcDNA3.1-ATG2B group.