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. 2020 Apr 26;12(8):7431–7447. doi: 10.18632/aging.103090

Figure 4.

Figure 4

The effects of KU70 and WRN on reprogramming efficiencies. (A) The relative protein expressions of KU70, WRN and SIRT1 in reprogramming MEFs on D5 and D10 upon DOX treatment. (n=3; *: p<0.05 when compared with MEFs; one way ANOVA). (B) The relative protein expression levels of KU70 and WRN in 2°F MEFs without (Ctrl) or with (RSV) RSV treatment after DOX induction. (C) The relative number of colonies formed after transfection with Ku70 (si-Ku70) or Wrn (si-Wrn) siRNA with or without the addition of RSV during the reprogramming. (n=3; *: p<0.05 as compared to siRNA control (si-ctrl); #: p<0.05 when compared with or without the addition of RSV; one way ANOVA). (D) The relative protein expressions of WRN in 1° MEFs after transfecting with miR-135a precursor (pre-135a) or inhibitor (inh-135a) for 72h (n=4; *:p<0.05; t-test). (E) The relative number of colonies formed after co-transfection with miR-135a inhibitor (inh-135a) and Sirt1 siRNA (si-Sirt1) during the reprogramming. (n=6; *: p<0.05 as compared to co-transfection with miRNA inhibitor control (inh-ctrl) and siRNA control (si-ctrl); one way ANOVA).