Table 1. Cryo-EM data collection, refinement and validation statistics.
| M. musculus apoferritin (EMDB-10205) (PDB 6SHT) | |
|---|---|
| Data collection and processing | |
| Magnification | 150,000X |
| Voltage (kV) | 200 |
| Electron exposure (e–/Å2) | 28 |
| Defocus range (μm) | -0.8 to -1.6 |
| Pixel size (Å) | 0.96 |
| Symmetry imposed | O |
| Initial particle images (no.) | 211,177 |
| Final particle images (no.) | 95,733 |
| Map resolution (Å) | 2.73 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.73–3.21 (Relion) |
| 2.10–3.60 (ResMap) | |
| Refinement | |
| Initial model used (PDB code) | 3wnw |
| Model resolution (Å) | 2.76 |
| FSC threshold | FSC = 0.143 |
| Model resolution range (Å) | 2.76–2.99a |
| Map sharpening B factor (Å2) | -95.1618 |
| Model composition | |
| Non-hydrogen atoms | 1474 |
| Protein residues | 173 |
| Ligands | 74 |
| B factors (Å2) | |
| Protein | 34.8 |
| Ligand | 36.6 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.015 |
| Bond angles (°) | 1.524 |
| Validation | |
| Clashscore | 5.96 |
| Poor rotamers (%) | 2.5 |
| Ramachandran plot | |
| Favored (%) | 98.9 |
| Allowed (%) | 0.5 |
| Disallowed (%) | 0.6 |
aresolution range includes resolution values reported at FSC = 0.143 against half-maps, unprocessed, unmasked map and the final processed masked map (S4 Fig).