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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Exp Neurol. 2020 Mar 9;328:113282. doi: 10.1016/j.expneurol.2020.113282

Fig. 4. Respirometry test for Complex IV activity.

Fig. 4

Complex IV functionality was assessed by monitoring azide-sensitive neuronal OCR supported by the exogenous electron donor TMPD in the presence of the TMPD-reducing agent ascorbate and the Complex III inhibitor antimycin A. UK5099 (10 μM) or vehicle (CON) was added first, followed by 200 μM DNP and 10 mM pyruvate (DNP+Pyr) to induce maximal respiration. Anti A (1 μM) was injected ± TMPD/ascorbate (TMPD/Asc, 0.4 mM/8 mM) as indicated. Finally, azide (5 mM) was added to confirm the specificity of the assay. OCR values are means ± SD from three wells.