Figure 2: Repopulation of peripheral blood CD20⁺ B cells after renal allograft transplantation (n=40).

The absolute lymphocyte cell counts and subsets are analyzed by polychromatic flow cytometry, and a representative dot plots of gating strategy for B cell analysis (top). Profound T cell and B cell (CD3⁻CD20⁺) depletion is achieved with alemtuzumab induction followed by rapid repopulation to baseline level at 6 months, then significantly exceeding baseline values thereafter (bottom). The box borders indicate 75^th and 25^th percentile, and the line within box indicates median. Upper and lower whiskers represent 90^th and 10^th percentiles. The dots represent fifth and 95^th percentiles.