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. Author manuscript; available in PMC: 2021 Mar 1.

Published in final edited form as: Am J Transplant. 2019 Nov 13;20(3):653–662. doi: 10.1111/ajt.15639

Figure 3. — (a) Absolute numbers and frequencies over time of CD27⁻IgD⁺ naïve B cells showing rapid repopulation post–alemtuzumab induction. (b) Absolute numbers and frequencies over time of memory B cells, including CD27⁺IgD⁻ (switched), CD27⁺IgD⁺ (unswitched), and CD27⁻IgD⁻ (exhausted) memory subsets. Alemtuzumab induction and belatacept-based immunotherapy significantly inhibits the repopulation of memory B cells. (c) Analysis of naïve and memory B cells using Bm1-Bm5 classification based on CD38/IgD expression shows that repopulating B cells are arrested at the Bm2 stage, consisting largely of naïve B cells. The Bm2’ subset, considering mainly regulatory/transitional cells, increases significantly over time during B cell repopulation. The box borders indicate 75^th and 25^th percentile, and the line within box indicates median. Upper and lower whiskers represent 90^th and 10^th percentiles. The dots represent fifth and 95^th percentiles.

Figure 3. — (a) Absolute numbers and frequencies over time of CD27⁻IgD⁺ naïve B cells showing rapid repopulation post–alemtuzumab induction. (b) Absolute numbers and frequencies over time of memory B cells, including CD27⁺IgD⁻ (switched), CD27⁺IgD⁺ (unswitched), and CD27⁻IgD⁻ (exhausted) memory subsets. Alemtuzumab induction and belatacept-based immunotherapy significantly inhibits the repopulation of memory B cells. (c) Analysis of naïve and memory B cells using Bm1-Bm5 classification based on CD38/IgD expression shows that repopulating B cells are arrested at the Bm2 stage, consisting largely of naïve B cells. The Bm2’ subset, considering mainly regulatory/transitional cells, increases significantly over time during B cell repopulation. The box borders indicate 75^th and 25^th percentile, and the line within box indicates median. Upper and lower whiskers represent 90^th and 10^th percentiles. The dots represent fifth and 95^th percentiles.