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. 2020 Apr 30;11(1):524–535. doi: 10.1080/21655979.2020.1757224

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DNAJC3-AS1 affected miR-214-3p and its related signaling pathway. (a) Putative miR-214-3p binding and mutated sites in DNAJC3-AS1. (b) Dual-luciferase reporter assay was performed to determine the luciferase activity of 293 T cells. (c) The expression of miR-214-3p was evaluated after the down-regulation of DNAJC3-AS1 in LoVo cells. (d-f) The protein levels of LIVIN (d), p-IκB (e), IκB (e), p-P65 (f) and P65 (f) in LoVo cells were detected by western blot. Data is shown as mean ± SD, ** p < 0.01, *** p < 0.001.