Fig. 4.

Protein analysis of P-gp and BCRP expression in preterm delivery with (PTDC) and in the absence of chorioamnionitis (PTD). A) Representative P-gp (150 kD), BCRP (72 kDa) and ß-actin (42 kDa) immunoblots followed by placental P-gp and BCRP expression normalized to ß-actin (internal control); B) Representative immunohistochemistry of BCRP; and C) P-gp in PTDC and PTD placentae. Semiquantitative score of the intensity of BCRP staining in the syncytiotrophoblast in PTDC compared to PTD slides. Black arrows indicate BCRP or P-gp staining, predominantly in the cytoplasm and apical membrane of the syncytiotrophoblast. Magnification bars represent 20 urn. Statistical analysis: unpaired t-test. Data are presented as mean ± SEM (n=6/group).