Fig. 3. Partial protection from autoimmune diabetes in NOD mice treated with CPMV-derived particles displaying the p524opt peptide.

The survival curves of groups treated with CPMV-derived particles indicate partial protection from the onset of diabetes in mice treated twice with CPMV.p524opt (blue line) compared to all the other groups: wt CPMV (green line), p524opt synthetic peptide + IFA (red line), and p524 synthetic peptide + IFA (gray line). The single administration of CPMV.p524opt (yellow line) did not prevent the onset of diabetes. The x axis indicates the number of days after the first treatment (A). The histopathology of pancreatic islet cells was determined in subsets of the treated mice by counting infiltrated cells. After 5 and 10 weeks of treatment (5w and 10w) and at the end of the treatment, some animals were euthanized to characterize the progression of the disease; CPMV.p524opt is the animal group that received double treatment (B).