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. 2020 May 6;6(19):eaaz0295. doi: 10.1126/sciadv.aaz0295

Fig. 6. Effect of TBSV-derived particles on cytokine profiles and the proportion of CD25+ Foxp3+ cells.

Fig. 6

(A) The percentage of CD25+ Foxp3+ cells was determined in the lymph nodes of non-CIA mice and CIA mice treated with saline, dexamethasone, wild-type TBSV, TBSV.pLIP1, TBSV.pFADK2, pLIP1 + IFA, and pFADK2 + IFA on day 60 after immunization. Values represent means ± SEM (n = 3; except for non-CIA mice, where n = 2). (B) Levels (pg/ml) of TGFβ1 and IL10 were determined in the serum of non-CIA mice and CIA mice treated with saline, dexamethasone, wild-type TBSV, TBSV.pLIP1, TBSV.pFADK2, pLIP1 + IFA, and pFADK2 + IFA on day 60 after immunization. Serum values represent data pooled from five mice. (C) TNFα, IL17A, IL-1β, and IFNγ levels (pg/ml) were determined in the serum (S) and lymph node (LN)/joint (J) supernatants of non-CIA mice and CIA mice treated with saline, dexamethasone, wild-type TBSV, TBSV.pLIP1, TBSV.pFADK2, pLIP1 + IFA, and pFADK2 + IFA on day 60 after immunization. Values represent means ± SEM (n = 3). Serum values represent data pooled from five mice. Statistical significance was determined by ANOVA, followed by Bonferroni correction. Values of P < 0.05 were considered significant (*). Significance was established for CIA mice treated versus saline-treated + CIA.