Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (C. elegans) | unc-68(r1162) | C. elegans Genetics Center (University of Minnesota) | Strain TR2171 RRID:WB-STRAIN:WBStrain00034950 |
|
| Genetic reagent (C. elegans) | unc-68(r1161) | C. elegans Genetics Center (University of Minnesota) | Strain TR2170 RRID:WB-STRAIN:WBStrain00034949 |
|
| Strain, strain background (E. coli) | HB101 | C. elegans Genetics Center (University of Minnesota) | ||
| Strain, strain background (E. coli) | HT115 (DE3) | C. elegans RNAi Collection (Ahringer) from Source BioScience | Clones IV-9P08 (pmk-1), IV-4G23 (pmk-2), IV-4I01 (pmk-3), and I-1K04 (pop-1) | RNAi library |
| Genetic reagent (D. rerio) | ryr1a(z42) | Chagovetz et al., 2019 | ZFIN ID: ZDB-ALT-180925–10 | |
| Genetic reagent (D. rerio) | ryr1b(mi340) | Hirata et al. (2007) | ZFIN ID: ZDB-ALT-070928–1 | |
| Chemical compound, drug | The US Drug Collection | MicroSource Discovery Systems, Inc | Chemical library | |
| Chemical compound, drug | DiscoveryProbe Kinase Inhibitor Library |
APExBIO | Catalog #L1024 | Chemical library |
| Chemical compound, drug | Nemadipine-A | ChemBridge | Catalog #5619779 | |
| Chemical compound, drug | Optovin analog 6b8 | ChemBridge | Catalog #5707191 | |
| Chemical compound, drug | SB203580 | Sigma | Catalog #S8307 | |
| Chemical compound, drug | SB202190 | Sigma | Catalog #S7067 | |
| Cell line (M. musculus) | C2C12 myoblasts | ATCC | Catalog #CRL1772 | |
| Cell line (M. musculus) | Ryr1 KO C2C12 myoblasts | This paper | 2 bp deletion in exon 6 of Ryr1: c.497_498del, p.Val166GlyfsX3 | |
| Antibody | anti-Ryanodine receptor (Mouse monoclonal) | Developmental Studies Hybridoma Bank (University of Iowa) | Catalog #34C, RRID:AB_528457 |
WB (1:100) |
| Antibody | anti- beta actin (Mouse monoclonal) | Abcam | Catalog #ab8226 RRID:AB_306371 |
WB (1:5000) |
| Sequence-based reagent | ON-TARGETplus Mouse Mapk11 siRNA | Horizon Discovery (Dharmacon) | Catalog # J-050928-05-0010 |
Target sequence: 5’-AUGAGGAGAUGACCGGAUA-3’ |
| Sequence-based reagent | ON-TARGETplus Mouse Mapk12 siRNA | Horizon Discovery (Dharmacon) | Catalog # J-062913-05-0010 |
Target sequence: 5’-AAUGGAAGCGUGUGACUUA-3’ |
| Sequence-based reagent | ON-TARGETplus Mouse Mapk14 siRNA | Horizon Discovery (Dharmacon) | Catalog # J-040125-06-0010 |
Target sequence: 5’-GCAAGAAACUACAUUCAGU-3’ |
| Sequence-based reagent | ON-TARGETplus Non-targeting siRNA #1 | Horizon Discovery (Dharmacon) | Catalog # D-001810-01-05 |
Target sequence: 5’-UGGUUUACAUGUCGACUAA-3’ |
| Sequence-based reagent | Tbp forward primer | This paper | Mouse Tbp gene: ENSMUSG00000014767 | 5’-TGCTGCAGTCATCATGAG-3’ |
| Sequence-based reagent | Tbp reverse primer | This paper | Mouse Tbp gene: ENSMUSG00000014767 | 5’-CTTGCTGCTAGTCTGGATTG-3’ |
| Sequence-based reagent | Mapk11 forward primer | This paper | Mouse Mapk11 gene: ENSMUSG00000053137 | 5’-CCAGCAATGTAGCGGTGAACGAG-3’ |
| Sequence-based reagent | Mapk11 reverse primer | This paper | Mouse Mapk11 gene: ENSMUSG00000053137 | 5’-GCATGATCTCTGGCGCCCGGTAC-3’ |
| Sequence-based reagent | Mapk12 forward primer | This paper | Mouse Mapk12 gene: ENSMUSG00000022610 | 5’-CACTGAGGATGAACCCAAGGCC-3’ |
| Sequence-based reagent | Mapk12 reverse primer | This paper | Mouse Mapk12 gene: ENSMUSG00000022610 | 5’-CTCCTAGCTGCCTAGGAGGCTTG-3’ |
| Sequence-based reagent | Mapk14 forward primer | This paper | Mouse Mapk14 gene: ENSMUSG00000053436 | 5’-CAGCAGATAATGCGTCTGACGGG-3’ |
| Sequence-based reagent | Mapk14 reverse primer | This paper | Mouse Mapk14 gene: ENSMUSG00000053436 | 5’-GCGAAGTTCATCTTCGGCATCTGG-3’ |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Catalog #74104 |