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. 2020 May 5;4(2):026105. doi: 10.1063/1.5143779

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© Author(s).

2473-2877/2020/4(2)/026105/16

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FIG. 2. — Clustering of adhesion parameters reveals ECM-specific effects on cell shape. (a) Visualization of continuum of cell adhesion of MDA-MB-231 cells on different ECM substrates based on all 11 cell shape parameters with SPRING plots. (b) Plots showing localization of the ECM factor-dependent cell adhesion shape on the combined SPRING plot. Mean centered cell adhesion of MDA-MB-231 (c) and MDA-MB-468 (d) cells plated on plastic, Collagen I, Fibronectin, Tenascin C, or Collagen IV for 2 h. Each cell adhesion parameter and ECM factor are clustered by rank correlation and mean linkage.