Figure 6.

Universal behavior of the DSB dynamics. (A) Relationship between the DSB focus size (R_B) and nuclear DSB density (ρ_B) for the trajectories from all NCS experiments is shown, both in the interior and periphery. (B) Relationship between the long-term DSB mobility (A_L) and R_B for the trajectories from all NCS experiments is shown, both in the interior and periphery. (C) R_B as a function of ρ_B and A_L for the interior DSBs across all NCS concentrations (Fig. S5) is shown. Boxes with greater transparency present average over fewer DSBs. (D) A diagram illustrating the general relationship between DSB focus size (R_B), DSB density (ρ_B), local chromatin compaction (S_rel, I_ch), and DSB mobility A_L is given. (a)–(f) illustrate different situations: in a nucleus with low DSB density, smaller DSB foci located in a weakly compacted chromatin are most mobile (a), whereas larger DSB foci in a weakly compacted chromatin are less mobile (b), and large DSB foci located in a more condensed chromatin are least mobile (c). In a nucleus with high DSB density, large DSB foci located in a more compact chromatin have the lowest mobility (d), whereas small DSB foci in a more compact chromatin have a higher mobility (e), and small DSB foci located in less compact chromatin are the most mobile (f). This diagram hints at a close relationship between the physical properties of a DSB and its surrounding chromatin, both influencing DSB mobility. Error bars for (A) and (B) are shown in Fig. S5. To see this figure in color, go online.