Table 6.

Classifier metrics using nanoparticle trajectories to predict particle type and cellular internalization status (PS-COOH in cells, PS-COOH out of cells, PS-PEG in cells, PS-PEG out of cells) in microglial cell culture. From left to right: (1) sample size of training and test datasets after spatial checkerboard split, (2) log median predictor using diffusion coefficients at τ = 100 ms averaged over 9 μm × 9 μm windows, (3) neural network predictor trained with principal components from trajectory feature analysis using both individual and locally averaged geometric features, (4) neural network predictor trained with principal components from trajectory feature analysis excluding particles <10 px from cell surfaces using both individual and locally averaged geometric features, (5) neural network predictor trained with principal components from trajectory feature analysis excluding particles <20 px from cell surfaces using both individual and locally averaged geometric features, (6) neural network predictor limited to PS-COOH nanoparticles trained with principal components from trajectory feature analysis excluding particles <10 px from cell surfaces using both individual and locally averaged geometric features, (7) neural network predictor limited to PS-PEG nanoparticles trained with principal components from trajectory feature analysis excluding particles <10 px from cell surfaces using both individual and locally averaged geometric features

	Sample size		Median predictora		Neural networka		Neural networka, b		Neural networka, c		Neural networka,c,d		Neural networka,c,e
	Training	Test	Training	Test	Training	Test	Training	Test	Training	Test	Training	Test	Training	Test
COOH out of cells	65 458	65 174	0.157	0.170	0.838	0.699	0.898	0.719	0.907	0.734	0.984	0.926	—	—
COOH in cells	48 825	49 832	0.207	0.208	0.935	0.787	0.966	0.804	0.977	0.837	0.982	0.852	—	—
PEG out of cells	56 517	57 097	0.159	0.154	0.946	0.847	0.965	0.850	0.973	0.865	—	—	0.985	0.979
PEG in cells	9810	9334	0.276	0.210	0.623	0.412	0.709	0.434	0.754	0.439	—	—	0.766	0.724
Avg/tot	180 610	181 437	0.200	0.185	0.836	0.686	0.885	0.702	0.903	0.719	0.983	0.889	0.875	0.852

^aPredictions performed using locally averaged features.

^bTrajectories within 10 pixels of a cell surface were excluded from predictions.

^cTrajectories within 20 pixels of a cell surface were excluded from predictions.

^dPredictor limited to PS-COOH nanoparticles to predict cell uptake status.

^ePredictor limited to PS-PEG nanoparticles to predict cell uptake status.