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NS overexpression protects HuH7 cells from drug-induced DNA damage. (A) Western blots of parental (WT) HuH7 cells and three NSOE lines overexpressing hemagglutinin (HA)-tagged human NS (NS-HA) from an EF1α promoter. Tub, α-tubulin. (B) DNA damage response of WT and NSOE cells to 5-FU, OXP, DRB, or olaparib. Y-axis shows γH2AX+ cell percentages. (C) Baseline mitotic activities of WT and NSOE cells determined by Ki67+ cell percentages. Bars represent means (± SEM); asterisks, see Fig.1.
