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. 2020 May 6;11:2228. doi: 10.1038/s41467-020-16070-6

Fig. 2. NR2 loser clones are eliminated by apoptosis via the TNF>JNK signalling axis.

Fig. 2

a Confocal images of wing discs that were immunostained with anti-phospho-JNK. Scale bar 50 μm. b Confocal images of wing discs that were immunostained with Phalloidin and anti-MMP1. Scale bar 50 μm. See Supplementary Data Table for genotypes. c Heterotypic clonal analysis. Schematic representation of the TNF>JNK signalling pathway. The indicated genes-of-interest (goi) were knocked down or misexpressed (UAS-bsk-DN or UAS-p35,UAS-Diap1) in GFP-marked clones as depicted in Fig. 1b (left panel). Clones are marked by GFP (green). Scale bar 100 μm. Quantification of the heterotypic competition assay. Diagram (error bars) shows the average occupancy of the indicated RNAi/over-expression clones per wing pouch ± SD. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.1 by two-tailed Mann–Whitney nonparametric U-test. n depicts the number of wing discs. Experiments were repeated four independent times. See Supplementary Table 1 for genotypes.