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. 2020 May 6;10:7630. doi: 10.1038/s41598-020-64893-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of PKS17 in DS68530 and 4xKO-B. (A) Schema showing the strategy used for integrating the IPNS promoter in front of Pc21g16000. (B) The initial molecule produced by PKS17 is the naphthopyrone YWA1, which was quantified by LC-MS in fermentation broth of the indicated strains after 3 and 5 days. (C) Expression of Pc21g16000 quantified by means of qPCR on day 3 of growth in SMP. The gene is not expressed in DS68530 and 4xKO-B and expression when replacing the promoter is unchanged between DS68530-PKS17-OE and 4KO-B-PKS17-OE, as seen by the difference in the similar Δct.