Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 6;10:7656. doi: 10.1038/s41598-020-64601-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In-vitro observation of human foetal RPEs and HEK293T cells devoid of melanin by PS-OCT. Digital photograph (a), stereomicroscopic image (b), and microscopic image (c) of a foetal human retinal pigment epithelial (hRPE) sheet in a transwell presenting a cobblestone-like appearance with pigmented cells. The sheet was used after 71 days of seeding in a transwell. En face map of polarization-sensitive optical coherence tomography (PS-OCT) intensity imaging (left) and entropy imaging(right) of the hRPE sheet (d). PS-OCT intensity (left) and entropy (right) of cross-sectional B-scan images of the transwell membrane only (e), human embryonic kidney (HEK) 293T cells on the membrane as a negative control (no melanin containing cells) (f), and the hRPE sheet on collagen gel on the membrane (g). In (d–f), arrowheads indicate a transwell membrane with high intensity and high entropy; arrows indicate presence of cells; asterisks indicate collagen gel. Scale bar, 50 µm.