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Preparation of the DNA-linked ADC. Reaction conditions: a) ABF (3 eq.) in PBS 1×, pH 7.5, 30 min, 25 °C; b) ON-BCN (2.9 eq.), 20 h, 25 °C; c) TCEP (100 eq.) in PBS 1×, pH 7.5, 2 h, 37 °C; d) APN-VC-MMAE (1.2 eq.) in 1/1 of DMSO/PBS 1×(pH 7.5, 5 mM EDTA), 16 h, 25 °C; e) in PBS 1×, pH 7.5, 20 min, 25 °C; f) BCN-VC-MMAE (4 eq.), 18 h, 25 °C.
