Skip to main content
. 2020 May 6;10:7649. doi: 10.1038/s41598-020-64507-1

Table 2.

Primers used in RT-PCR reactions.

Primer Name Sequence (5′-3′)
EF1A_346F CGTCAAGGAACTTCGTCGTG
EF1A_456R CACAGGGGTGTATCCGTTG
TSWVL_4382F GCATGAAYTGGTTRCAAGGC
TSWVL_4493R CAGAGTGCACAATCCATCTAG
Emilia_5.8 S_rRNAF GTGTGAATTGCAGAATCCCGT
Emilia_5.8 S_rRNAR CATGTGACGCCCAGGCA

The elongation factor 1 alpha (EF1A) primers were used as a reference sequence in Thrips tabaci. TSWVL primers were used to target the L RNA sequence for TSWV, and the Emilia_5.8S primers were based on the reference sequence for Emilia sonchifolia.