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. 2020 May 6;10:7622. doi: 10.1038/s41598-020-64616-x

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© The Author(s) 2020, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative distribution of reads in sequence data generated by nanopore sequencing of DNA purified from the three selected blood cultures. The blood cultures were spiked with E. coli A2-39 (A and D), K. pneumoniae A2-37 (B and E) and E. coli A2-39 + K. pneumoniae A2-37 (C and F). Upper panel (A–C) show results obtained using Centrifuge and lower panel (D–F) show results based on BLAST search against the RefProk database that contains prokaryotic sequence data only. The “Others” group represents taxa with relative read counts below 1%. All results are based only on the first output file for each experiment from the MinION sequencing platform, containing 4000 reads (available after approximately 10 minutes of sequencing).