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. 2020 May 6;10:7641. doi: 10.1038/s41598-020-63393-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stress-responsive genes and a persistent increase in Lcn2 expression induced by MALP-2. (A) HC11 cells were treated with LPS (10 µg/ml) or MALP-2 (10 ng/ml) for the indicated time periods and RNA samples prepared and quantified for IL-6, TNFα and cyclophilin mRNAs by RT-qPCR. The values for IL-6, TNFα were normalized to the cyclophilin values for the same sample and the zero time point for each gene set was used to normalize the results for all time points of that set. (B) HC11 cells were treated with 10 ng/ml MALP-2 for the time periods shown (MALP-2, Lcn2) or treated with MALP-2 for 4 hours then the cells were washed and the medium changed to the same medium lacking MALP-2 [MALP-2 (4 h), Lcn2]. Lcn2, TNFα and cyclophilin mRNA levels were quantified by RT-qPCR and normalized to cyclophilin. (C) HC11 cells were treated with MALP-2 at various concentrations for 12 h. Lcn2 and cyclophilin mRNAs were then quantified by RT-qPCR. All values were normalized to the average control value from cells treated with vehicle.