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. 2020 May 6;10:7662. doi: 10.1038/s41598-020-64713-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential effects of miR-145 and AFAP1-AS1 on the regulation of breast cancer cell viability and colony formation in vitro and tumor formation in vivo. (A) Cell viability assay. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h, and cell viability was analyzed by MTT assay. (B) Colony formation assay. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to a colony formation assay. The graph shows the summarized data from the assay. (C) Cell viability assay. MDA-MB-231 cells were transiently transfected with pSilencer-NC plus ASO-NC, pshR-AFAP1-AS1 plus ASO-NC, or pshR-AFAR1-AS1 plus ASO-miR-145 for 48 h, and cell viability was assessed by the MTT assay. (D) Colony formation assay. MDA-MB-231 cells were transiently transfected with pSilencer-NC plus ASO-NC, pshR-AFAP1-AS1 plus ASO-NC, or pshR-AFAR1-AS1 plus ASO-miR-145 for 48 h before analysis with a colony formation assay. The graph shows the summarized data from the assay. (E) Changes in mouse body weight in nude mouse tumor cell xenograft model. (F) Changes in tumor volume in nude mouse tumor cell xenograft model. (G) Photographs of tumor xenografts. **p < 0.05 and ***p < 0.01.