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. 2020 May 6;10:7662. doi: 10.1038/s41598-020-64713-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction of AFAP1-AS1 with miR-145 and miR-145 with MTH1 in breast cancer cells. (A) qRT-PCR. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to qRT-PCR analysis of miR-145 expression. (B) Luciferase assay. MDA-MB-231 cells were transiently transfected with pmirGLO/AFAP1-AS1 3′-UTR, miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to Luciferase assay. The right panel shows the results of the Luciferase assay with mutated pmirGLO/AFAP1-AS1 3′-UTR transfection. (C) Luciferase assay. MDA-MB-231 cells were transiently transfected with pmirGLO/NUDT1 3′-UTR, miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to Luciferase assay. The right panel shows the results of the Luciferase assay with mutated pmirGLO/NUDT1 3′-UTR transfection. **p < 0.05 and ***p < 0.01.