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. 2020 May 6;10:7662. doi: 10.1038/s41598-020-64713-x

Figure 5.

Figure 5

Interaction of miR-145 with AFAP1-AS1 and miR-145 with MTH1 in breast cancer cells. (A) qRT-PCR. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to qRT-PCR analysis of AFAP1-AS1 expression. (B) qRT-PCR. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to qRT-PCR analysis of MTH1 expression. (C) Western blot. MDA-MB-231 cells were transiently transfected with miR-145 mimics, negative control, ASO-miR-145, or ASO-NC for 48 h and subjected to Western blot analysis of MTH1 expression. (D) qRT-PCR. MDA-MB-231 cells were transiently transfected with three different AFAP1-AS1 siRNAs and subjected to qRT-PCR analysis of AFAP1-AS1 expression. (E) qRT-PCR. MDA-MB-231 cells were transiently transfected with AFAP1-AS1-3 siRNA and then subjected to qRT-PCR analysis of miR-145 expression. **p < 0.05 and ***p < 0.01.