Table 1.
Endophytic fungi isolated from healthy T. granulosa leaves and access numbers searched in GenBank for identification
| N° sp | Strain | Identification | Isolate | Access on GenBank* | ||
|---|---|---|---|---|---|---|
| ITS | TUB | TEF-1 | ||||
| 1 | Tg 06 | Phyllosticta capitalensis | CBS 100175 CBS 123404 CBS 119720 | FJ538320.1 (97.5) FJ538333.1 (97.5) KF206178.1 (97.5) | - | FJ538378.1 (100) FJ538391.1 (98.9) FJ538398.1 (98.4) |
| 2 | Tg 012 | Diaporthe cf. hevea 1 | CBS 852.97 | KC343116.1 (99.2) | KC344084.1 (98.7) | KC343842.1 (99) |
| 3 | Tg 013 | Colletotrichum karstii | CMM 3797 | KC702990.1 (99.6) | KC992339.1 (98) | N/A |
| 4 | Tg 014 | Diaporthe sp | CBS 114015 | KC343010.1 (95.6) | KR936132.1 (87.3) | - |
| 5 | Tg 029 | Colletotrichum jiangxiense | LF 684 LF 687 | KJ955198.1 (98.2) KJ955201.1 (98.2) | KJ955345.1 (99) KJ955348.1 (99) | N/A |
| 6 | Tg 032 | Diaporthe oxe | CBS 133186; LGMF915; CBS 133187 | KC343164.1 (99.2) KC343166.1 (99.2) KC343165. 1 (98.1) | KC344132.1(99.1) KC344134.1 (98.1) KC344133.1 (97.3) | KC343890.1 (100) KC343892.1 (100) KC343891.1 (100) |
| 7 | Tg 042 | Diaporthe oxe | CBS 133186; LGMF915; CBS 133188 | KC343164.1 (99.2) KC343166.1 (99.2) KC343165.1 (99.2) | KC344132.1 (98.4) KC344134.1 (97.3) KC344133.1 (97.7) | KC343890.1 (100) KC343892.1 (100) KC343891.1 (100) |
| 8 | Tg 046 | Colletotrichum karsii | CBS 128500 | JQ005202.1 (99.7) | JQ005636.1 (100) | N/A |
| 9 | Tg 048 | Diaporthe sp | BRIP 45669 | NR147537.1 (96.6) | - | KT459451.1 (87.4) |
| 10 | Tg 049 | Nigrospora sp. | P39E2 | JN207335.1 (99.7) | - | - |
| 11 | Tg 051 | Colletotrichum karstii | CBS 128500 | JQ005202.1 (99.7) | JQ005636.1 (100) | N/A |
| 12 | Tg 055 | Colletotrichum siamense | BPDI2 | FJ972613.1 (97.6) | FJ907438.1 (98.5) | N/A |
| 13 | Tg 057 | Diaporthe endophytica | LGMF948 | KC343072.1 (99.6) | KC344040.1 (97.5) | KC343798.1 (100) |
| 14 | Tg 070 | Diaporthe sp | BRIP57892a | NR147534.1 (95.5) | - | JQ954663.1 (85.3) |
| 15 | Tg 073 | Diaporthe paranaensis | CBS 133184 | KC343171.1 (97.5) | KC344139.1 (98.2) | KC343897.1 (97) |
| 16 | Tg 077 | Diaporthe sp | CBS 122.21 | NR152456.1 (96.7) | KP189344.1 (97.3) | JQ954663.1 (85.2) |
| 17 | Tg 079 | Xylaria grammica | 5151 | JQ862665.1 (96.3) | JX868535.1 (92.1) | N/A |
| 18 | Tg 111 | Colletotrichum jiangxiense | LF 684 LF 687 | KJ955198.1 (98.6) KJ955201.1 (98.6) | KJ955345.1 (100) KJ955348.1 (100) | N/A |
| 19 | Tg 134 | Fusarium circinatum | CBS 405.97 | KC464617.1 (99.7) | KM232080.1 (99) | KM231943.1 (97.4) |
| 20 | Tg 154 | Cercospora sp | CPC24809 | NR147294.1 (97) | - | JX143412.1 (100) |
| 21 | Tg 168 | Xylaria berteroi | YMJ 90112623 | KC473562.1 (100) | AY951763.1 (98.1) | KC465407.1 (99) |
| 22 | Tg 178 | Diaporthe sp | CBS122676 | NR111846.1 (96.8) | KC344009.1 (93.9) | JX862537.1 (89.2) |
| 23 | Tg 180 | Diaporthe cf. hevea 1 | CBS 852.97 | KC343116.1 (99.1) | KC344084.1 (99.6) | KC343842.1 (98.9) |
| 24 | Tg 184 | Diaporthe sp | BRIP57892a | KJ197276 (97.9) | KR936132.1 (92.7) | JQ972716.1 (85.5) |
| 25 | Tg 189 | Diaporthe sp | BRIP 57892a | NR147534.1 (96) KX197977.1 (100) | KR936132.1 (92.2) | KJ197241.1 (90.5) |
| 26 | Tg 190 | Diaporthe melonis | CBS507.78 CBS435.87 FAU640 CMT41 | NR103700.1 (97.4) FJ889447.1 (97.4) | KC344020.1 (95.6) | KJ590764.1 (98.3) |
| 27 | Tg 203 | Diaporthe melonis | CBS507.78 CBS435.87 FAU640 CMT41- | NR103700.1 (95) FJ889447.1 (95) | KC344038.1 (95.1) | KJ590764.1 (96.7) |
| 28 | Tg 207 | Diaporthe melonis | CBS507.78 CBS435.87 FAU640 CMT41 | NR103700.1 (96.6) FJ889447.1 (96.5) | KC344084.1 (82.5) | KJ590764.1 (96.1) |
| 29 | Tg 215 | Colletotrichum sp | BCC38876 | NR111637.1 (98) | KP890114.1 (98.37) | N/A |
| 30 | Tg 233 | Hypoxylon investiens | STMA 14058 | KU604568.1 (96.1) | KU159528.1 (99.6) | N/A |
*Values indicate percent sequence identity compared with GenBank
-Gene not sequenced
ITS, internal spacer regions transcribed from the nrDNA and interconnecting the 5.8 SnrDNA; TUB, partial beta-tubulin gene; TEF1, partial translation stretching factor 1-alpha gene; N/A, gene not located in the database for the genre in question; CBS, Fungi Biodiversity Center, Utrecht, The Netherlands; LGMF, cultural heritage of the Laboratory of Genetics of Microorganisms of the Federal University of Paraná, Curitiba, Brazil; LF, Fang Liu’s work collection, housed in CAS, China; Collection CMM culture of phytopathogenic fungi “Prof. Maria Menezes,” Recife, Brazil; STMA, HZI culture collection, Helmholtz Center for Infection Research, Braunschweig, Germany