Figure 4.

Quantification of γH2AX/H2AX levels in MEF cells after Sirt1 depletion or incubation with various inhibitors by western blot analysis (original western blots: Supplementary Figure 4) with anti-γH2AX and anit-H2AX. γH2AX/H2AX levels are decreased in Sirt1 KO cells and upon addition of EX-527 (10 μM), S1th 10 (30 μM), S1th 12 (20 μM), or S1th 13 (12 μM). The selective Sirt2 inhibitor SirReal2 (30 μM) does not affect γH2AX/H2AX levels. Addition of Camptothecin increases DNA damage in cells (A) γH2AX/H2AX levels without Camptothecin; (B) γH2AX/H2AX levels after addition of Camptothecin (10 μM). The experiments were performed at least three times each. *p < 0.05, **p < 0.01, ***p < 0.001.