Table 3.

Inhibition of sirtuins 1, 2 3 and 5 by thiocyanate analgues of 4th round of virtual screening (S1th 10-13) and PR-619.

Compound	IC50 [μM] or % inhibition @ 50 μM
	Sirt1	Sirt2	Sirt3	Sirt5**
S1th 10	23 ± 6.0	n.i.*	n.i.	n.i.
S1th 11	6.3 ± 0.8	n.i.	n.i.	n.i.
S1th 12	5.9 ± 1.4	n.i.	n.i.	n.i.
S1th 13	5.2 ± 1.0	n.i.	n.i.	n.i.
PR-619	2.7 ± 0.2	36%	n.i.	28%

^*n.i., no inhibition (<10%),

^**inhibition tested @ 10 μM.