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. 2020 Apr 30;11:578. doi: 10.3389/fmicb.2020.00578

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Copyright © 2020 Tagliaferri, Guimarães, Pereira, Vilela, Horz, dos Santos and Mendes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the CRISPR-Cas9-based interference with the bla_TEM–₁ gene in a clinical isolate of E. hormaechei 4962 and K. variicola 68AI. (A,B) Disk diffusion susceptibility test on E. hormaechei 4962 grown on Mueller-Hinton agar with measurement of inhibition zones. The level of resistance could be significantly reduced for aztreonam (ATM), cefotaxime (CTX), and ceftriaxone (CRO) along with acquired resistance against chloramphenicol (CHL). Note that only for ATM the intermediate sensitivity category was reached according to CLSI-definitions. (C,D) Disk diffusion susceptibility test on K. variicola 68AI grown on Mueller-Hinton agar with measurement of inhibition zones showing minor resistance reductions for ceftazidime (CAZ) and cefepime (FEP), along with acquirement of full resistance against CHL. Horizontal bars represent the mean of duplicate disk diffusion tests. *p < 0.05; **p < 0.01; ****p < 0.0001.