Table 2.
Biomass, MCA, and PA of the crude enzyme from A. oryzae DRDFS13
| Sample | Day | pH mean ± SD |
Biomass in mg GlcN/gdfs | MCA(U/mL) mean ± SD |
PA(U/mL) mean ± SD |
Ratio MCA/PA |
|---|---|---|---|---|---|---|
| Blank | _ | 3.93 ± 0.01a | – | NDc | 0.00 ± 0.00f | ND |
| Aspergillus oryzae DRDFS13 | 0 | 3.91 ± 0.01a | 10.15 ± 0.11c | NDc | 0.00 ± 0.00f | ND |
| 1 | 3.78 ± 0.00a | 12.43 ± 2.02c | NDc | 0.00 ± 0.00f | ND | |
| 2 | 3.90 ± 0.09a | 19.77 ± 8.20a | NDc | 100.24 ± 6.88e | ND | |
| 3 | 3.85 ± 0.01a | 16.64 ± 6.48b | NDc | 129.07 ± 1.64d | ND | |
| 4 | 3.90 ± 0.02a | 11.43 ± 0.18c | 63.76 ± 6.62b | 196.39 ± 3.12b | 0.32 | |
| 5 | 3.94 ± 0.01a | 11.30 ± 0.04c | 79.60 ± 3.16a | 172.26 ± 10.53c | 0.46 | |
| 6 | 4.02 ± 0.20a | 10.96 ± 0.71c | 70.20 ± 1.20b | 231.24 ± 1.01a | 0.30 | |
| Chymosin | _ | – | – | 2181.81 ± 0.00 | 281.75 ± 24.79 | 7.74 |
Chymosin, commercially produced pure enzyme used as positive control; ND, not determined (if milk did not clot within 40 min); MCA, milk-clotting activity (U/mL); PA, protease activity (U/mL); ratio, the ratio of MCA/PA; SD, standard deviation; mean, average of two measurements; different letters (a, b, c, d) designate significantly different means as determined by Duncan multiple mean comparison test (P < 0.05)