Table 5.
Experimental design used in the RSM studies of three independent variables with 3 center points for MCP production by A. oryzae DRDFS13 under SSF
| Run order | A pH |
B Casein conc. |
C Temp. |
MCA (U/mL) | |
|---|---|---|---|---|---|
| Actual value | Predicted value | ||||
| 1. | 4.00 | 0.50 | 25.00 | 134.85 | 137.58 |
| 2. | 7.00 | 0.50 | 25.00 | 107.99 | 93.31 |
| 3. | 4.00 | 5.00 | 25.00 | 131.18 | 115.50 |
| 4. | 7.00 | 5.00 | 25.00 | 79.34 | 71.23 |
| 5. | 4.00 | 0.50 | 40.00 | 83.05 | 62.72 |
| 6. | 7.00 | 0.50 | 40.00 | 0.00 | 18.44 |
| 7. | 4.00 | 5.00 | 40.00 | 15.95 | 40.62 |
| 8. | 7.00 | 5.00 | 40.00 | 14.55 | −3.65 |
| 9. | 2.98 | 2.75 | 32.50 | 132.24 | 104.20 |
| 10. | 8.02 | 2.75 | 32.50 | 49.49 | 29.74 |
| 11. | 5.5 | −1.03 | 32.50 | 69.78 | 85.54 |
| 12. | 5.5 | 6.53 | 32.50 | 30.57 | 48.40 |
| 13. | 5.5 | 2.75 | 19.89 | 101.95 | 129.93 |
| 14. | 5.5 | 2.75 | 45.11 | 0.00 | 4.01 |
| 15. | 5.5 | 2.75 | 32.5 | 56.65 | 66.97 |
| 16. | 5.5 | 2.75 | 32.5 | 72.89 | 66.97 |
| 17. | 5.5 | 2.75 | 32.5 | 59.54 | 66.97 |
| 18. | 5.5 | 2.75 | 32.5 | 56.94 | 66.97 |
| 19. | 5.5 | 2.75 | 32.5 | 79.18 | 66.97 |
| 20. | 5.5 | 2.75 | 32.5 | 63.22 | 66.97 |
MCA, milk-clotting activity (U/mL); casein conc., casein concentration (%); and temp., temperature in °C