. 2020 Mar 24;51(2):571–584. doi: 10.1007/s42770-020-00243-y

Table 6.

Inhibition study for the crude enzyme from A. oryzae DRDFS13

Sample	Fermentation Time (days)	MCA(U/mL) Mean ± SD	Inhibition study Residual MCA (%)
Sample	Fermentation Time (days)	MCA(U/mL) Mean ± SD	Pep A (1 mM)	I.A (10 mM)	EDTA (10 mM)	PMSF (10 mM)
Aspergillus oryzae DRDFS13	0	ND^c	ND^d	ND^d	ND^d	ND^d
	1	ND^c	ND^d	ND^d	ND^d	ND^d
	2	ND^c	ND^d	ND^d	ND^d	ND^d
	3	ND^c	ND^d	ND^d	ND^d	ND^d
	4	63.76 ± 6.62^b	0.00^d	97.14^c	94.56^c	95.78^c
	5	174.61 ± 3.18^a	0.00^d	72.94^b	69.82^b	76.22^b
	6	170.22 ± 1.21^a	0.00^d	84.19^ab	89.52^a	74.80^b

D, not determined; MCA, milk-clotting activity (U/mL); Pep A, pepstatin A; I.A, iodoacetamide; EDTA, ethylenediaminetetraacetic acid; PMSF, phenyl-methane sulphonyl fluoride; SD, standard deviation; mean is average of two measurements; different letters (a, b, c, for MCA (U/mL) designate significantly different means as determined by Duncan multiple mean comparison test (P < 0.05); different letters (a, b, c,d for PepA, I.A, EDTA, and PMSF) designate significantly different means as determined by Duncan multiple mean comparison test (P < 0.05)