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. 2019 Oct 26;51(2):773–778. doi: 10.1007/s42770-019-00161-8

Fig. 2.

Fig. 2

UV treatment at 4.8 × 103 μJ/cm2 to viable, killed, and a mixture of viable and killed cells of Escherichia coli MTCC 3221 for 3 min. UV treatment at 4.8 × 103 μJ/cm2 for 3 min resulted in complete inhibition of DNA amplification from killed cells by qPCR (a). Amplification curves for PMA-treated viable, killed, and a mixture of viable and killed cells of Escherichia coli MTCC 3221 at 4.8 × 103 μJ/cm2 for 3 min (b). Each bar represents the average Cq value of a triplicate experiment ± standard deviation. ∆Rn, fluorescence intensity change; NTC, no template control