Table 1.
Information of the primary antibodies used in this study
| Anitibodya | Specificitya | Type | Item numbers | Dilutionb | Source |
|---|---|---|---|---|---|
| β-actin | β-actin | Polyclonal | #3700 | 1:10,000 WB | Cell signaling |
| IRS-1 | Total IRS-1 | Polyclonal | ab52167 | 1:1000 WB/1:200 IHC | Abcam |
| p-IRS-1(Tyr612) | p-IRS-1 at Tyr612 | Polyclonal | 44-816G | 1:1000 WB | Thermo |
| Polyclonal | sc-17195-R | 1:50 IF/1:50 IHC | Santa Cruz | ||
| PI3K-p85α | Total PI3 Kinase p85 | Polyclonal | #4292 | 1:1000 WB | Cell signaling |
| p-PI3K-p85α (Tyr607) | p-PI3K-p85α at Tyr607 | Polyclonal | ab182651 | 1:1000WB | Abcam |
| Akt | Total Akt | Monoclonal | #4691 | 1:1000 WB | Cell signaling |
| p-Akt (Ser473) | p-Akt at Ser473 | Monoclonal | #4060 | 1:1000 WB | Cell signaling |
| GSK3β | Total GSK3β | Monoclonal | #9832 | 1:1000 WB/ | Cell signaling |
| p-GSK3β(Ser9) | p-GSK3β at Ser9 | Monoclonal | #5558 | 1:1000 WB/1:200 IHC/1:100 IF | Cell signaling |
| DCX | Doublecortin(C-18), Microtubule-associated protein in migrating and differentiating neurons | Polyclonal | sc-1084 | 1:1000 WB/1:50 IHC/1:50 IF | Santa Cruz |
| Neuro D | Neuro D(N-19), Helix–loop–helix protein expressed in differentiating neurons | Polyclonal | sc-8066 | 1:1000 WB/1:100 IHC | Santa Cruz |
| Brdu | 5-Bromodeoxyuridine | Monoclonal | ab6326 | 1:100 IF | Abcam |
| Aβ | Beta amyloid | Monoclonal | 6E10 | 1:1000 WB/1:200 IHC | Covance |
ap phosphorylated, bWB western blot, IHC immunohistochemistry, IF immunofluorescence