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. 2020 Apr 30;10:192. doi: 10.3389/fcimb.2020.00192

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Copyright © 2020 Niu, Wang, Yang, Chen, Zhang, Chen, Zhuge, Li, Cheng, Xu, Chu, Ren, Zhang and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ca²⁺/calcineruin pathway was involved in carvacrol-induced C. albicans apoptosis. (A) Effects of CsA on carvacrol-treated C. albicans growth were analyzed, and the data was shown as mean ± SD (n = 5). (B) Metacaspase activation was examined upon pretreated with CsA for 2 h. The histogram showed the percentage of stained cells, and the data were exhibited as mean ± SD (n = 3). (C) The calcineurin mutant cmp1Δ/Δ, and calcium-scavenger BAPTA, were used to observe metacaspase activity. The histogram showed the percentage of stained cells, and the data were expressed as mean ± SD (n = 3). *P < 0.05 vs. the YPD group; #P < 0.05 vs. 247 μg/ml Car group.