FIGURE 5.
Pore formation caused by MV-associated bacteriocins. Increase in red wavelength fluorescence from LIVE/DEAD stain indicates peptides in the LabIP-induced L. acidophilus ATCC 53544 supernatant (A) and those associated with MVs (B) create pores in the L. delbrueckii cell wall and/or membrane. (C) Representative fluorescence microscopy images of LIVE/DEAD-stained L. delbrueckii show an increase in red-fluorescing cells after incubation with MVs from LabIP-induced L. acidophilus, while L. delbrueckii treated with MVs from non-induced cultures show a minimal number of red-fluorescing cells.