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. 2020 Apr 30;11:710. doi: 10.3389/fmicb.2020.00710

FIGURE 5.

FIGURE 5

Pore formation caused by MV-associated bacteriocins. Increase in red wavelength fluorescence from LIVE/DEAD stain indicates peptides in the LabIP-induced L. acidophilus ATCC 53544 supernatant (A) and those associated with MVs (B) create pores in the L. delbrueckii cell wall and/or membrane. (C) Representative fluorescence microscopy images of LIVE/DEAD-stained L. delbrueckii show an increase in red-fluorescing cells after incubation with MVs from LabIP-induced L. acidophilus, while L. delbrueckii treated with MVs from non-induced cultures show a minimal number of red-fluorescing cells.