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. 2020 Apr 30;11:710. doi: 10.3389/fmicb.2020.00710

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Copyright © 2020 Dean, Rimmer, Turner, Phillips, Caruana, Hervey, Leary and Walper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optiprep purification of MVs. (A) Following fractionation MV concentration (particles/mL) was determined using NanoSight from 1:100 diluted fractions in PBS. (B) Coomassie stain of SDS-PAGE gel of the 10 Optiprep fractions. (C) Spot-on-lawn of L. delbrueckii following Optiprep fractionation; control (ctrl) is the input MVs prior to fractionation.