TABLE 3.
Quantitative mass spectrometry of putative bacteriocins in MVs.
ATCC 53544 | NCFM | Targeted peptide | Precursor ion m/z (Da) | MW | Charge | Retention time | Uninduced | Induced |
locus | locus | sequence | (min) | sample | sample | |||
CGZ81_00665 | LBA1791 | VGVGWAAEAR | 508.27 | 1015.53 | 2 | 32.46 | NA | 4.64E+06 |
CGZ81_00695 | Lactacin B | STAWGAVSGAATGTAVG QAVGALGGALFGGSMGVIK | 1059.89 | 3176.63 | 3 | 32.97 | 5.07E+04 | 2.15E+06 |
CGZ81_00710 | LabIP | APISGYVGR | 460.25 | 918.48 | 2 | 27.67 | NA | 4.97E+06 |
GLWENLSNIFK | 660.88 | 1319.69 | 2 | 51.15 | 1.71E+06 | 3.40E+09 | ||
CGZ81_00715 | LBA1802 | RDMILVALPHAVGPDGM PGSGR | 749.39 | 2245.15 | 3 | NA | NA | NA |
DMILVALPHAVGPDGM PGSGR | 697.36 | 2089.05 | 3 | 42.69 | NA | 5.57E+05 | ||
AIGSIPPWRPNWWK | 569.98 | 1706.89 | 3 | NA | NA | NA | ||
CGZ81_00730 | LBA1805 | LLGPDGMEGSMGGSTG GIQSFR | 1077.5 | 2153.02 | 2 | 39.48 | NA | 8.14E+05 |
RHFPGFGR | 409.21 | 816.38 | 2 | NA | NA | NA |
“NA” indicates that the tryptic peptide was not detected. Values for uninduced and induced samples are the calculated Area Under the Curve (AUC).