FIGURE 2.

Mg²⁺ does not prevent CPP2-induced calcification in hVSMCs. (A) Alizarin red staining and (B) Ca²⁺ deposition quantification show that CPP2-induced calcification is not prevented by 2.0 mmol/L Mg²⁺ after 24 h and 4 days. (C) Gene expression related to calcification after CPP2 treatment was measured after 2 days (black bars) and 4 days (white bars) and remained unchanged by Mg²⁺. (D) Protein expression of OPN, SM22α and MGP was measured and quantified. Data are shown as fold changes compared with the day-matched controls and presented as the mean of three separate experiments, consisting of three replicate cultures ± SD. *P < 0.05.