FIGURE 4.

Mg²⁺ dose-dependently inhibits the transition from CPP1 to CPP2 and does not affect CPP2 morphology after nucleation. (A) Absorbance of the CPP mixture containing different Mg²⁺ concentrations was measured at 570 nm as a readout for CPP1 (∼0.05 AU) to CPP2 (∼0.15 AU) transition. This graph is representative of five independently executed experiments each consisting of three replicates. Data are expressed as mean ± SD. A final concentration of 2.0 mmol/L Mg²⁺ after CPP2 (E–G) formation did not alter morphology (SEM) or composition (EDX) compared with control CPP2 (B–D). A TEM picture was inserted in B to confirm CPP2. Scale bars correspond to 300 nm (white) and 500 nm (black).