Table 2.
Primers targeting different gene regions were used to detect the papillomavirus CePV1v, BPV1, BPV2, and the parapoxvirus OV and PVNZ from 27 skin lesions' samples.
| Gene | Acronym | Primers | Product (bp) | References |
|---|---|---|---|---|
| Putative viral envelope antigen (OV) | B2L | PPP-1 5′-GTCGTCCACGAGCAGCT-3′ PPP-4 5′-TACGTGGGAAGCGCCTCGCT-3′ |
595 | (38) |
| Granulocyte–macrophage-colony-stimulating factor/ interleukin-2 inhibition factor (OV) | GIF | GIF 5 5′-GCTCTAGGAAAGATGGCGTG-3′ GIF 6 5′-GTACTCCTGGCTGAAGAG CG-3′ |
408 | (39) |
| Viral interleukin 10 ortholog (OV) | vIL-10 | vIL-10-3 5′-ATGCTACTCACACAGTCGCTCC-3′ vIL-10-4 5′-TATGTCGAACTCGCTCATGGCC-3′ |
300 | (39) |
| Vascular endothelial growth factor gene of OV NZ-2 like (OV) | VEGF-E | VEGF_forNZ2 5′ATGARGTTGCTCGTCKGCATAC-3′ VEGF_rev1NZ2 5′-CGTCTTCTGGGCGGCCTTGT-3′ VEGF_rev2NZ2 5′-CTTCGGCGCCGTCTAGGC-3′ |
399 | This study |
| Vascular endothelial growth factor gene of (OV) | VEGF-E | GF1 5′-GCGGGATCCGCCATGAAGTTGCTCGT-3′ GF2 5′-GCGGAATTCCTAGCGGCGTCTTCTGG-3′ |
399 | (40) |
| Vascular endothelial growth factor gene of (PVNZ) | VEGF-E | 5′-TTTGGCGCGCCAGAGACTTCTAATACAGTGTAGCG-3′ 5′-TCACCCGAACGCGTACGTCTTGGAGGCATAG-3′ |
447 | (41) |
| Vascular endothelial growth factor NZ7 like gene (OV) | VEGF-E | GF3 5′-GCGGGATCCACGATGAAGTTAACAGC-3′ GF4 5′-GCGGAATTCTTATCGTCTAGGTTCCCTA-3′ |
450 | (40) |
| minor capsid protein (CePV1v) | L2 | CePVL2F 5′-TAGACTACTACTACCTGTGACACAC-3′ CePVL2R 5′-TGGTCACAGGTGTAGGTGGCA-3′ |
250 | (33) |
| major capsid protein (CePV1v) | L1 | CePVL1F5731 5′-TATTTGCCACCTACACCTGTGAC-3′ CePVR7253 5′-CAGCTGGACAGCTCATTAG-3′ |
1522 | (33) |
| E5 oncoprotein (BPV1 and BPV2) | E5 | 5′B1/2-E5: 5′-CACTACCTCCTGGAATGAACATTTCC-3′ 3′B1/2-E5: 5′-CTACCTTWGGTATCACATCTGGTGG-3′ |
499 | (42) |
| major capsid protein (BPV1) | L1 | BPV1estL1f 5′-TGATGGGCACACAGTTGATTTGTAC-3′ BPV1estL1r 5′-GGTGCAGTTGACTTACCTTCTGT-3′ |
1621 | This study |