Fig. 2.

Dpp3 gene expression and enzymatic activity are effectively shut down in the Dpp3 KO mouse model. (A) Representative RT-PCR for Dpp3 gene expression in several tissues obtained from WT (odd numbers) and Dpp3 KO (even numbers) mice. (B) qPCR of Dpp3 gene on splenocytes and total bone from WT and Dpp3 KO mice (n = 3 per genotype). (C) Evaluation of DPP3 enzymatic activity in WT. (E) Dpp3 KO mice in protein extracts from tail biopsies and total bone (n ≥ 3 per genotype). The Arg-Arg-2-naphthylamide peptide was used as reaction substrate. The enzymatic activity was quantified by spectrophotometric analysis. Data are presented as the mean ± SE. ***p < .001. (D) Representative Western blot analysis of Dpp3 protein in total bone tissue from WT and Dpp3 KO mice. β-actin = loading control; Gapdh = housekeeping control.